How To Use The FOXP3 Antibody In Research

The FOXP3 Antibody is a 431 amino acid protein and also a member of the forkhead/winged-helix family. It is required for normal immune homeostasis. It can be stably and constitutively expressed at higher levels in the CD25+CD4 regulatory positive T-cells. It can also be found at low levels in the CD4 positive and CD35 negative cells. It is not seen in CD4 negative and CD8 positive T-cells. It can be a specific marker of regulatory T-cells and could be a master regulatory gene.

More Information

The FOXP3 antibody doesn’t have a clone, and the immunogen is the synthetic peptide that was derived from the c-terminus of the human protein. The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight is 50kDa.

Applications

The FOXP3 antibody can be used with Immunohistochemistry applications. To prepare your specimen, use a Formalin-fixed or paraffin-embedded tissue. Deparaffinized slides should always be used, and you can clean them with graded alcohols, xylene or one of its alternatives.

When using the concentrated version of the product, you’ll need to dilute the antibody using a ratio of 1:200, though this is an estimate. Your protocols may require different dilution options. If you do need to use the standard ratio, you can always purchase the pre-diluted immunogen.

To retrieve the antigen, you will need to boil the tissue sections in a 1mM EDTA with a pH of 8.0 for 10 minutes. You should allow it to cool to room temperature for at least 20 minutes before proceeding. You will also need to incubate it at room temperature for 30 minutes before visualization.

The positive control will be the tonsil with cellular localization occurring in the nucleus.

The FOXP3 antibody can be used a variety of ways. Visit Spring Bioscience to purchase the right amount for your research needs.

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