In almost all types of research with regards to testing and study of genetics, DNA sequencing plays a major role in clinical and research studies. For large scale testing requirements, next-generation sequencing or NGS is fast, effective and provides parallel sequencing to develop large sequencing volumes in very small periods of time.
However, not all applications require this large volume sequencing capacity. Many labs use the traditional method of Sanger sequencing, which provides one forward and one reverse read at a time. While this may be slower, these systems, including the Applied Biosystems sequencer models, offering 99.99% accuracy, a factor that is critical for research applications.
Advantages of Sanger Sequencing
The use of the Applied Biosystems sequencer, which uses Sanger sequencing, is considered the “gold standard” for sequencers due to the accuracy of the method.
While it may not offer the mass volume or massively parallel sequencing, these systems have high turnaround times and are also known for high throughput rates, creating a significant workflow through the lab. Different models of the Applied Biosystems sequencer will have different workload capacities, and it is important to consider your needs.
Another factor to consider as a benefit to the Sanger sequencing technology offered by the Applied Biosystems equipment is the low cost. This is the most cost-effective sequencing method, which is often a factor for labs across the country to consider. The high accuracy also figures into the cost, eliminating the need for verification runs.
The cost of the equipment for the Applied Biosystems Sanger sequencers does vary based on the model and the capacity of the system. For very budget-friendly options, consider choosing a used or refurbished model, which can cut the cost significantly without reducing any of the features or functionality found on newer models.
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