Labs and testing facility around the world use the Applied Biosystems sequencer models, most commonly the 3130 and 3130XL for genetic analysis and sequencing of both human as well as animal genetics.
These compact, highly efficient and effective systems provide precise data, allow for multiple operations with one system while also providing an easy to use operator interface that is one of the most intuitive options on the market.
Using the Equipment
The Applied Biosystems sequencer, regardless of the model selected, is very easy to set up and integrate with the current lab systems. The components include the genetic analyzer, which is a standalone system. This is connected to a Windows-based computer. A standard monitor, keyboard, and mouse are all is required.
The systems are designed to accommodate a wide range of sequencing run types from ultra rapid to long read. Different capillary arrays will be used in the various types of runs, with shorter lengths of capillaries used for the ultra fast, rapid and fast runs and longer used for standard the long read testing.
Fragment analysis also uses different capillary lengths as well as polymer types and run times. Becoming familiar with the requirements for all types of sequencing and analysis options will be critical for users.
Factors to Consider
The Applied Biosystems sequencer comes with the option to use barcode readers for additional accuracy in testing. The data collection software for the system is easy to configure and use, just requiring a basic system review to turn on the applications as the software will come with all applications off.
From this point, once the applications are turned on, the menu is very easy to use. Subfolders contact all necessary information and data, allowing technicians to set up the system, retrieve data and to control the instrument through a very simple and practical interface.